Journal: bioRxiv

Article Title: TGFβ blocks IFNα/β release and tumor rejection in spontaneous mammary tumors

doi: 10.1101/336990

Figure Lengend Snippet: (a) Spont-PyMT mice were injected i.p. with DMXAA and then sacrificed after 3 hours and 24 hours to measure mRNA levels of cytokines and chemokines in tumors. The relative expressions compared to DMSO treated mice are shown. Each dot corresponds to one tumor. Cumulative data from CTRL: n= 6 mice and DMXAA: n= 6 mice, from 3 independent experiments, are shown. Tukey’s Multiple Comparison Test. (b and c) The relative expression of these cytokines/chemokines, compared to PBS (CTRL) injected mice, were measured in Spont-PyMT and Trans-PyMT target lesion 3 hours after (b) cGAMP (25μg i.t.) injection (Spont-PyMT mice: n= 3 CTRL and n= 3 cGAMP; Trans-PyMT mice: n= 5 CTRL and n= 13 cGAMP) or (c) LPS (50μg i.p.) injection (Spont-PyMT mice: n= 3 CTRL and n= 3 LPS; Trans-PyMT mice: n= 5 CTRL and n= 10 LPS). In b and c , results are expressed as mean ± s.e.m.

Article Snippet: LPS (1x 50 μg) was injected i.p., cGAMP (InvivoGen, 1 × 25 μg) was injected i.t. and mice were sacrificed 3 hours later.

Techniques: Injection, Comparison, Expressing